Objectives

·         To determine the trypanocidal activity of new formulations nanostructured for T. cruzi in vitro.

·         To determine the toxicity to mammalian cells lines and primary cultures.

·         To evaluate the in vitro efficacy of new formulations for T. cruzi.

Description of work (possibly broken down into tasks), and role of participants.

·         Task 1. Set up of laboratory conditions for culturing epimastigote, trypomastigote and amastigote forms of different stocks of T. cruzi and for primary cultures of cardiomyocytes and neural glia cells.

·         Task 2. In Vitro cytotoxicity.

·         Task 3. Epimastigote and trypomastigote inhibition assays.

 

·         Task 4. Amastigote growth inhibition assay.